A Fresh Start

I came to the school and work back from a short Spring Break. I was worry about soy beans that I planted them during spring break because college was closed Thursday through Sunday for Spring Break and I was not able to water them. Fortunately, my Soy Beans grew.

 

I purified DNA of eight different bacteria that are used in the Biosciences department laboratory and run PCR (Polymerase Chain Reaction) for them during spring break. On Monday, I used Agarose Gel Electrophoresis technique for the separation of bacteria’s DNA fragments.Then, I cultured Agrobacteria in the Brain Heart Infusion, 0.5 ml broth. Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials.(bd.com)

On Tuesday, I set up another gel for bacteria 5-8 because I couldn’t get a good result on Monday. Meanwhile, I started to transfer my soy beans from Vermiculite which is ideal for the germination of seeds to the garden potting soil mix but before that I had to infect my soy beans with Agrobacterium. I didn’t like to infect my Soy beans because I planted and grown them by myself but I didn’t have any choice, I had to do that.  Thus, I choose three soy beans that grown most and infected them in three different ways with a fresh Agrobacterium that I cultured in Brain Heart Infusion broth. For the first soybeans, I cut its stem with a razor blade a little bit then I injected Agrobacterium to that part with a syringe. For the second Soy beans, I injected Agrobacterium directly to the stem with a syringe.

 For the third one, I just mixed Agrobacterium with potting soil around the Soy bean’s stem and place it in the separate plant saucer. For the reason that Agrobacterium that I mixed with soil would go through water and I didn’t want other soy beans observe water that is polluted by Agrobacterium. I’ll monitor my three infected soy beans to see which way of infection would be more effective and accountable.